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The sections were incubated with 10 nM [ 68 Ga]Ga-DO3A-Exendin-4 either alone or pre- incubated (10 min in PBS + 1% BSA), with 1 M of DO3A-Exendin-4 (generated by custom in-house Solid Phase Peptide Synthesis (SPPS) by Sano), for block- ing, in 150 ml of PBS + 1% Bovine Serum Albumin (BSA) for 60 min at room temperature (RT)

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Melanotan 1 (MT-1) and Melanotan 2 (MT-2) are synthetic peptides developed to stimulate melanin production, aiding sunless tanning and skin protection